(A) In Dlx1::CreER/Ai14 reporter mice, cells are permanently labeled by tdTomato only if 4-OHT has been administered when the Dlx1 promoter is active, i.e., the neurogenetic phase of striatal progenitors.
(B) 4-OHT was administered at successive developmental times, separated by a single day.
(C) Striatum of 4-OHT pulse-labeled mice was assessed at maturity. Coronal sections in upper panels were stained for tdTomato (red), CalDAG-GEFI (CDG1, green), and MOR1 (blue). Lower panels show individual channels in grayscale.