Table 3.
Inheritance and genetic crosses-single-generation outcrosses
| Cross number | Outcross, P1 phenotype | F1, n= | F1 phenotype, % yellow | F1 phenotype, % green | P-value, 2-side exact binomial test |
|---|---|---|---|---|---|
| y × y | Expected ratio: 100:0 | ||||
| 1 | y, y | 346 | 100 | 0 | 1.0 |
| 2 | y, y | 200 | 100 | 0 | 1.0 |
| 3 | y, y | 69 | 100 | 0 | 1.0 |
| G × y | Expected ratio: 50:50 | ||||
| 4 | G, y | 37 | 43 | 57 | 0.511 |
| 5 | G, y | 22 | 45 | 55 | 0.831 |
| 6 | G, y | 68 | 44 | 56 | 0.396 |
| 7 | G, y | 31 | 55 | 45 | 0.720 |
| 8 | G, y | 15 | 53 | 47 | 1.0 |
| 9 | G, y | 112 | 59 | 41 | 0.72 |
| 10 | G, y * | 20 | 55 | 45 | 0.82 |
| G × y | Expected ratio: 0:100 | ||||
| 11 | G, y* | 28 | 0 | 100 | 1.0 |
| 12 | G, y * | 77 | 0 | 100 | 1.0 |
| G × G | Expected ratio: 25:75 | ||||
| 13 | G, G * | 48 | 31 | 69 | 0.319 |
| 14 | G, G * | 50 | 32 | 68 | 0.25 |
| 15 | G, G * | 165 | 35 | 65 | 0.007 |
| 16 | G, G * | 32 | 56 | 44 | <0.0001 |
| G × G | Expected ratio: 0:100 | ||||
| 17 | G, G | 108 | 14 | 86 | 0.007 |
| 18 | G, G | 99 | 15 | 85 | 0.005 |
| 19 | G, G | 599 | 0 | 100 | 1.0 |
| 20 | G, G | 25 | 0 | 100 | 1.0 |
| 21 | G, G | 293 | 2 | 98 | 0.9795 |
| 22 | G, G | 91 | 2 | 98 | 0.9795 |
| 23 | G, G | 137 | 5 | 95 | 0.949 |
| 24 | G, G | 19 | 5 | 95 | 0.949 |
Individuals of yellow (‘y’) and green (‘G’) color morphs were crossed in either yellow × yellow, yellow × Green or Green × Green combinations. Number of offspring (F1) resulting from each parental cross (P1), as well as the percentage of each color morph observed in the fifth instar per family. Families are numbered and grouped by the best fit two-allele, single-gene expected model which they were compared to using a two-sided exact binomial test. A P-value of > 0.05 indicates that the observed ratio is not significantly different from a ratio one would expect from a single, two-allele gene. Families that were one generation of a multigenerational cross are noted by an ‘*’; complete multigenerational family lineage can be seen in Table 4.