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. Author manuscript; available in PMC: 2020 Aug 18.
Published in final edited form as: Cell Rep. 2020 Aug 4;32(5):107989. doi: 10.1016/j.celrep.2020.107989

Figure 1. Mitochondrial Defects in GR80 Transgenic Fly Muscle.

Figure 1.

(A) Images showing abnormal wing posture phenotypes in Mhc-Gal4>GR80 flies. The bar graph shows age-dependent progression of the wing posture phenotype (n = 90 flies, 3 biological repeats). The error bars signify standard deviation (SD) in this and all of the other figures.

(B) Monitoring of mitochondrial morphology in thoracic flight muscle of Mhc-Gal4>GR80 flies expressing a mito-GFP reporter. The images are representative of 3 independent samples.

(C) TEM images of IFM mitochondria of control (Mhc-Gal4/w-) and Mhc-Gal4>GR80 flies at lower (1,000×) and higher (6,000×) magnifications. The graphs show quantification (n = 5 sections from 3 independent samples).

(D) Mitochondrial respiratory chain activities in GR80 compared to control (Mhc-Gal4/w-) flies. The data represent 1-time experiment.

(E) Quantification of ROS levels measured with DCFH fluorescence in GR80 fly muscle (n = 3).

(F) Mito-SOX staining of mitochondrial ROS levels in control and GR80 flies expressing mito-GFP. The graph shows quantification (n = 3).

*p < 0.05 and **p < 0.01, 1-way ANOVA test followed by Student-Newman-Keuls (SNK) test plus Bonferroni correction. See also Figure S1.