Figure 4.

Exosomal miR‐133b act as a mediator for intercellular communication. The expression of miR‐133b in the medium of 5637 and T24 cells treated with RNase (3 μg/ml) alone or combined with Triton X‐100 (0.3%) for 20 minutes (A). Transmission electron microscopy image of exosomes derived from the serum of patients and controls. Scale bars represent 100 nm (B). Western blotting analysis showing the presence of CD63 and CD81 in exosomes (C). Confocal microscopy image showing the internalization of PKH67‐labeled exosomes derived from 5637 and T24 cells for three hours, green represents PKH67, and blue represents nuclear DNA staining by DAPI (D). *P < .05