Figure 3.

Exosomal miR‐204‐5p inhibits CRC cells proliferation and induces apoptosis. A, The expression of miR‐204‐5p in CRC cells cocultured with 293T‐derived exosomes. B‐C, Exosomal miR‐204‐5p (miR‐204 EXO) inhibited CRC cell proliferation (B) and colony formation ability(C) compared with the control exosomes (GFP EXO). D, CRC cells cocultured with miR‐204 EXO or GFP EXO for 24 hours were treated with 6 µg/mL 5‐FU for 48 hours. Flow cytometry showed that miR‐204‐5p EXO enhanced 5‐FU‐induced apoptosis in CRC cells. E, Exosomal miR‐204‐5p (miR‐204‐5p EXO) inhibited cell proliferation in breast cancer (MCF‐7 and MDB‐231), lung cancer (A549), gastric cancer (SGC‐7901), and glioma (U251) cells. The cell proliferation activity was measured by CCK‐8 at 24 hours and 72 hours after coculturing with EXO. *P < .05, **P < .01, ***P < .001. Shown are mean ± SEM from three independent experiments