Figure 4.

Exosomal miR‐204‐5p increases sensitivity of cancer cells to chemotherapy. A, The protein expression of miR‐204‐5p target genes (RAB22A and Bcl2) in CRC cells incubated with the exosomes of 293T‐GFP (GFP EXO) and 293T‐miR‐204 cells (miR‐204 EXO) for 48 hours were tested by Western blot. B‐E, Exosomal miR‐204‐5p increased the sensitivity of cancer cells to 5‐FU. The IC50s of LoVo (1.254 vs 3.047 µg/mL), HCT116 (0.674 vs 1.664 µg/mL), MCF‐7(1.200 vs 4.312 µg/mL) and SGC‐7901 (8.230 vs 25.460 µg/mL) cocultured with exosomal miR‐204‐5p were significantly lower than those of their corresponding controls (P < .01). Shown are mean ± SEM from three independent experiments