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. 2020 Aug 18;15(8):e0232917. doi: 10.1371/journal.pone.0232917

Fig 6. Treatment with cRGDfK inhibits TGF-β1-induced expression of EMT markers.

Fig 6

(A) Serum-deprived A549 cells were treated with TGF-β1 (5 ng/mL) or its combination with cRGDfK for 72 h. The protein expression of TGF-β1-mediatead EMT markers was detected by Western blot. Actin was used as a loading control. (B‒E) Serum-deprived A549 cells were pretreated with cRGDfK for 3 h, and then treated with TGF-β1 (5 ng/mL) for 48 h (B and C) or 1 h (D and E). The mRNA expression of CDH2 and Vim (B and C) or Snail and Twist (D and E) was measured by qRT-PCR using GAPDH as an internal control. Experiments were performed in triplicate. Data represent the mean ± SD of raw results. # p < 0.01 versus control, * p < 0.05, ** p < 0.01 versus treatment with TGF-β1 only.