Fig 4. Meiotic DNA repair is not affected in pch-2^E253Q mutants but crossover assurance is.

A. Images of meiotic nuclei stained with DAPI and antibodies against RAD-51. Color and grey scale images are provided. B. Timecourse of the average number of RAD-51 foci per nucleus in wildtype, pch-2^E253Q and pch-2Δ mutant germlines. Error bars indicate 2XSEM. C. Images of meiotic nuclei stained with DAPI and antibodies against GFP. D. Percentage of nuclei with five GFP::COSA-1 foci in wildtype animals, pch-2^E253Q and pch-2Δ mutants. Error bars indicate 95% confidence intervals. Significance was assessed by performing two-tailed Fisher exact tests. A *** indicates a p value < 0.0001.