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. 2020 Aug 11;7(4):ENEURO.0172-20.2020. doi: 10.1523/ENEURO.0172-20.2020

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Copyright © 2020 Cooper et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — Farnesene favors high-sensitivity nAChRs in neuro-2a cells. Representative neuro-2a cells transfected with α4-mCherry, α4-GFP or α6-GFP, and β2wt nAChR subunits to produce (A) α4-mCherryα6-GFPβ2 nAChRs or (C) α4-mCherryα4-GFPβ2 nAChRs. Scale bar, 10 μm. Mean NFRET pixel count (B₁, D₁) and NFRET percentage (B₂, D₂) treated as control or with 0.5 μm farnesene for (A) α4-mCherryα6-GFPβ2 nAChRs or (C) α4-mCherryα4-GFPβ2 nAChRs. All data are mean ± SEM; *p < 0.05, ****p < 0.001; unpaired t test. Exact p values are given in Results. Dots within bars represent the values from individual cells within the designated treatment group; n > 30 cells per condition.