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. 2020 Aug 11;7(4):ENEURO.0172-20.2020. doi: 10.1523/ENEURO.0172-20.2020

Table 1.

Statistical tests and results

Figure Type of test Interaction/main effect Statistical data
1, 2 Two-way
ANOVA
Sex × drug F(5,88) = 3.045, p = 0.0140
1, 2 Two-way ANOVA Sex F(1,88) = 10.55, p = 0.0016
1, 2 Two-way ANOVA Drug F(5,88) = 12.21, p < 0.0001
1A1 One-way ANOVA F(3,30) = 5.98, p = 0.0025
1A1 Post hoc
Tukey
Saline vs 0.1 mg/kg; p = 0.0065
1A1 Post hoc
Tukey
Saline vs 1.0 mg/kg; p = 0.9997
1A1 Post hoc
Tukey
Saline vs 10 mg/kg; p = 0.9770
1A1 Post hoc
Tukey
0.1 vs 1.0 mg/kg; p = 0.0203
1A1 Post hoc
Tukey
0.1 vs 10 mg/kg; p = 0.0047
1A1 Post hoc
Tukey
1.0 vs 10 mg/kg; p = 0.9680
1A2 One-way ANOVA F(3,25) = 9.81, p = 0.0002
1A2 Post hoc
Tukey
Saline vs 0.1 mg/kg; p < 0.0001
1A2 Post hoc
Tukey
Saline vs 1.0 mg/kg; p = 0.0126
1A2 Post hoc
Tukey
Saline vs 10 mg/kg; p = 0.0402
1A2 Post hoc
Tukey
0.1 vs 1.0 mg/kg; p = 0.4631
1A2 Post hoc
Tukey
0.1 vs 10 mg/kg; p = 0.2245
1A2 Post hoc
Tukey
1.0 vs 10 mg/kg; p = 0.9669
1B1 Unpaired
t test
Saline vs farnesene (males), p = 0.111
1B2 Unpaired
t test
Saline vs farnesene (females), p = 0.801
2A One-way ANOVA F(2,23) = 8.506, p = 0.0017
2A Post hoc
Tukey
Saline vs nicotine; p = 0.0173
2A Post hoc
Tukey
Saline vs nicotine + farnesene; p = 0.0022
2A Post hoc
Tukey
Nicotine vs nicotine + farnesene; p = 0.4280
2B One-way ANOVA F(2,25) = 13.04, p = 0.0001
2B Post hoc
Tukey
Saline vs nicotine; p = 0.2916
2B Post hoc
Tukey
Saline vs nicotine + farnesene; p = 0.0001
2B Post hoc
Tukey
Nicotine vs nicotine + farnesene; p = 0.0041
3C1 Unpaired
t test
[α4, p = 0.2239], [α6, p = 0.9065], [α4α6, p = 0.6259]
3C2 Unpaired
t test
p = 0.1268
3C3 Unpaired
t test
p = 0.1227
3D1 Unpaired
t test
[α4, p = 0.0515], [α6, p = 0.4842], [α4α6, p = 0.0653]
3D2 Unpaired
t test
p = 0.5572
3D3 Unpaired
t test
p = 0.6156
4A1 Unpaired
t test
p = 0.6343
4A2 Unpaired
t test
p = 0.7225
4B1 Unpaired
t test
p = 0.5285
4B2 Unpaired
t test
p = 0.0480
5B1 Unpaired
t test
p < 0.0001
5B2 Unpaired
t test
p < 0.0001
5D1 Unpaired
t test
p = 0.0149
5D2 Unpaired
t test
p < 0.0001
7E1 Unpaired
t test
p = 0.0002
7E2 Unpaired
t test
p < 0.0001
8B One-way ANOVA F(2,12) = 23.05, p < 0.0001
8B Post hoc
Tukey
5 μm farnesene vs 500 μm farnesene; p = 0.1823
8B Post hoc
Tukey
5 μm farnesene vs 100 μm nicotine; p < 0.0001
8B Post hoc
Tukey
500 μm farnesene vs 100 μm nicotine, p = 0.0031
8E1 Unpaired
t test
p < 0.0001
8E2 Unpaired
t test
p < 0.0001