Fig 5. CHK-265 treatment results in reduced pro-inflammatory cytokines, chemokines, and immune cell infiltration in musculoskeletal tissue.

Four-week-old WT male and female C57BL/6 mice were administered 100 μg of CHK-265 prior to infection with RRV. (A-F) Ipsilateral quadriceps muscles were harvested 3 or 7 dpi and analyzed for cytokine and chemokine levels. Bars indicate median values (n = 8–9 per group; two experiments; Mann-Whitney test; *P < 0.05, **P < 0.01). (G) At 7 dpi, ipsilateral quadriceps muscles were digested, single cell suspensions were stained for CD45⁺ leukocytes, Ly6C^hi monocytes, F4/80^hiLy6C^hi monocyte-derived macrophages, F4/80^hiLy6C^lo tissue resident macrophages, NK1.1⁺CD3^- NK cells, Ly6G⁺ neutrophils, CD4⁺ T cells, or CD8⁺ T cells, and analyzed by flow cytometry. Bars indicate median values (n = 9 per group; two experiments; Mann-Whitney test; **P < 0.01, ***P < 0.001, ****P < 0.0001).