Skip to main content
. 2020 Aug 18;751:141750. doi: 10.1016/j.scitotenv.2020.141750

Fig. 1.

Fig. 1

SARS-CoV-2 specific RNA fragment detected by (A) M-gene and (B) RdRP RT-qPCR in the aqueous phase of untreated and treated municipal sewage. Error bars indicate one standard deviation of duplicate samples. Results of a single PCR measurement are shown for M-gene. For M-gene RT-qPCR, CT values for the standard ranged between 8.6 (standard 1 = 108) and 38.4 (standard 6 = 102). Values of tested wastewater above CT 39 were considered negative for SARS-CoV-2. Standard curve was calculated using Bio-Rad CFX Manager software with E = 61.4%, R2 = 0.952 and slope = −4.813. Results of two independent PCR measurements of the same samples are shown for RdRp. For RdRP RT-qPCR CT values for standard range between 18 (standard 1 = 108) and 37 (standard 6 = 102). Values of tested wastewater above CT 38 were considered negative for SARS-CoV-2. Standard curve was calculated using Bio-Rad CFX Manager software with E = 96.5%, R2 = 0.990 and slope = −3.408.