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. 2020 Aug 18;751:141750. doi: 10.1016/j.scitotenv.2020.141750

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Fig. 6 — Viral outgrowth assay setup. Caco-2 cells were inoculated with either wastewater samples or with SASR-CoV-2 containing cell culture supernatant (positive control) or culture medium only (negative control). Cells were investigated daily by light microscopy for the appearance of a cytopathic effect caused by replication-competent SARS-CoV-2 infection for up to 10 days. The experiment was performed in a single replicate for four different wastewater samples (P2, P5, P11, P12) from April 8th 2020.