Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Caenorhabditis elegans) | coq-2 | WormBase | WBGene00000762 | |
| Strain, strain background (Caenorhabditis elegans) |
N2 | Caenorhabditis Genetics Center (CGC) | N2 | Wild-type |
| Strain, strain background (Caenorhabditis elegans) |
coq-2 (syb1715) | This paper | PHX1715 |
coq-2∆6a
Supplementary file 1 |
| Strain, strain background (Caenorhabditis elegans) |
coq-2 (syb1721) | This paper | PHX1721 |
coq-2∆6e
Supplementary file 1 |
| Strain, strain background (Escherichia coli) | OP50 | Caenorhabditis Genetics Center (CGC) | OP50 | |
| Sequence-based reagent | sgRNAs used in this study | This paper | Supplementary file 1 | |
| Sequence-based reagent | PCR primers used in this study | This paper | Supplementary file 1 | |
| Chemical compound, drug | Potassium cyanide (KCN) | Sigma-Aldrich | 60178–25G | Stock solution: 50 mM in PBS buffer |
| Other | Hexane, HPLC grade | Sigma-Aldrich | 650552–1L | |
| Other | Acetonitrile, LC-MS grade | Fisher Scientific | A955-4 | |
| Other | Ubiquinone-3 standard | Campbell et al., 2019 | ||
| Other | Ubiquinone-9 standard | Sigma-Aldrich | 27597–1 MG | |
| Other | Rhodoquinone-9 standard | Roberts Buceta et al., 2019 | Isolated from Ascaris suum | |
| Software, algorithm | Whippet | Sterne-Weiler et al., 2018 | RRID:SCR_018349 | https://github.com/timbitz/Whippet.jl |
| Software, algorithm | HISAT2 | Kim et al., 2019 | RRID:SCR_015530 | https://daehwankimlab.github.io/hisat2/ |
| Software, algorithm | UCSF Chimera | Resource for Biocomputing Visualization and Informatics Pettersen et al., 2004 | RRID:SCR_004097 | http://plato.cgl.ucsf.edu/chimera/ |
| Software, algorithm | MODELLER | University of California at San Francisco Webb and Sali, 2016 | RRID:SCR_008395 | https://salilab.org/modeller/ |
| Software, algorithm | Image analysis pipeline | Spensley et al., 2018 | https://github.com/fraser-lab-UofT/acute_assay | |
| Software, algorithm | Python | Python | RRID:SCR_008394 | https://www.python.org/ |
| Software, algorithm | NIS-Elements | Nikon | RRID:SCR_014329 | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| Software, algorithm | ImageMagick | ImageMagick | RRID:SCR_014491 | https://imagemagick.org/ |
| Software, algorithm | BioFormats | OME - Open Microscopy Environment | RRID:SCR_000450 | https://docs.openmicroscopy.org/bio-formats/5.7.1/users/comlinetools/index.html |