Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Tribolium castaneum) | TcMCU | IDT Inc | ||
| Gene (Tribolium castaneum) | TcEMRE | IDT Inc | ||
| Gene (Homo sapiens) | HsMICU1 | IDT Inc Genewiz, Inc. |
||
| Gene (Homo sapiens) | HsMCIU2 |
IDT Inc
|
||
| Cell line (H. sapiens) | Expi293F | Sigma | A14527 | |
| Cell line (H. sapiens) | HEK-293T-MICU1-KO | Kamer and Mootha, 2014 | DOI: 10.1002/embr.201337946 | |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine | Avanti Polar Lipids | 850757 | |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) | Avanti Polar Lipids | 840457 | |
| Chemical compound, drug | 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (sodium salt) | Avanti Polar Lipids | 710335 | |
| Chemical compound, drug | Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K) | Polysciences, Inc | 23966–1 | |
| Chemical compound, drug | Sodium Butyrate | Sigma | 8451440100 | |
| Chemical compound, drug | n-Dodecyl-β-D-maltopyranoside | Anatrace | O310S | |
| Chemical compound, drug | (2α,3β,5α,15β,25R)−2,15-dihydroxyspirostan-3-yl O-β -D-glucopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→4)-β-D-galactopyranoside |
Cayman Chemical Company | 14952 | |
| Chemical compound, drug |
Membrane Scaffold Protein 1D1 | Sigma | M6574 | |
| Chemical compound, drug | 5-Methyl-2-oxo-4-imidazo lidinehexanoic acid |
Sigma | D1411 | |
| Chemical compound, drug | Lipofectamine 3000 Transfection Reagent |
Thermo Fisher | L3000008 | |
| Chemical compound, drug | Calcium Green−5N, Hexapotassium Salt | Thermo Fisher | C3737 | |
| Software, algorithm | MotionCor2 | Zheng et al., 2017 | RRID:SCR_016499 | |
| Software, algorithm | CtfFind 4.1.10 | Rohou and Grigorieff, 2015 | RRID:SCR_016731 | |
| Software, algorithm | RELION 3.0 | Zivanov et al., 2018 |
http://www2.mrc-lmb.
cam.ac.uk/relion RRID:SCR_016274 |
|
| Software, algorithm | SerialEM | Glover, 2004 | RRID:SCR_017293 | |
| Software, algorithm |
cryoSPARC v2 | Structura Biotechnology |
https://cryosparc.com/
RRID:SCR_016501 |
|
| Software, algorithm | PHENIX | Adams et al., 2010 |
https://www.phenix-online.org/
RRID:SCR_014224 |
|
| Software, algorithm | COOT | Emsley et al., 2010 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
RRID:SCR_014222 |
|
| Software, algorithm | PyMOL | Schrödinger, 2020 |
https://pymol.org/2/
RRID:SCR_000305 |
|
| Software, algorithm | UCSF Chimera | Pettersen et al., 2004 |
https://www.cgl.ucsf.edu/chimera
RRID:SCR_004097 |
|
| Software, algorithm | GraphPad Prism 7 | GraphPad Software |
https://cryosparc.com/
RRID:SCR_016501 |
|
| Software, algorithm | Hole | Smart et al., 1996 | http://www.holeprogram.org | |
| Software, algorithm | UCSF ChimeraX | Goddardnet al. 2018 | https://www.cgl.ucsf.edu/chimerax/ | |
| Others | QUANTIFOIL R1.2/1.3 holey carbon grids | Quantifoil | ||
| Others | FEI Vitrobot Mark IV | FEI Thermo Fisher |