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. 2020 Aug 13;12:7251–7262. doi: 10.2147/CMAR.S248869

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© 2020 Wang et al.

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ZFAS1 interacted with miR-421 to modulate cell proliferation and apoptosis. (A) The putative binding site as well as the corresponding mutant region of miR-421 to ZFAS1. (B) Luciferase activity of MUT-ZFAS1 and WT-ZFAS1 reporter systems in 293T cells affected by miR-421 was detected using luciferase reporter assay. (C and D) The expression of miR-421 affected by ZFAS1 knocking-down or overexpression was determined in OSCC cells (C) and DDP-resistance OSCC cells (D) by qRT-PCR. Transfection of CAL-27/DDP and SCC-9/DDP cells with anti-miR-421 and si-UCA1 or si-UCA1 alone. (E and F) Cell growth was determined by CCK-8. (G and H) DDP sensitivity was detected by calculating IC50 value using CCK-8. (I) Cell apoptosis detected by flow cytometry analysis. (J) Analysis of caspase-3 activity. (K) Expressions of Bax and BCL2 detected by Western blot. ***P < 0.005, **P < 0.01, *P < 0.05.