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. 2020 Aug 14;12:7277–7289. doi: 10.2147/CMAR.S257311

Figure 4.

Figure 4

Knockdown of miR-770-5p reverses the suppressive effect of NEAT1 silencing on cisplatin resistance in cisplatin-resistant ovarian cancer cells. (A and B) The expression of miR-770-5p was measured in A2780/DDP and SKOV3/DDP cells transfected with si-NC, si-NEAT1 #2, si-NEAT1 #2 and anti-NC or anti-miR-770-5p by qRT-PCR. (C and D) Cell viability and IC50 of cisplatin (DDP) were detected in A2780/DDP and SKOV3/DDP cells transfected with si-NC, si-NEAT1 #2, si-NEAT1 #2 and anti-NC or anti-miR-770-5p after treatment of cisplatin for 48 h by MTT. (E and F) Cell viability was measured in A2780/DDP and SKOV3/DDP cells transfected with si-NC, si-NEAT1 #2, si-NEAT1 #2 and anti-NC or anti-miR-770-5p at 0, 24, 48 and 72 h by MTT. (G and H) Cell apoptosis was detected in A2780/DDP and SKOV3/DDP cells transfected with si-NC, si-NEAT1 #2, si-NEAT1 #2 and anti-NC or anti-miR-770-5p at 72 h by flow cytometry. (I and J) The protein levels of Bcl-2, Bax and Cleaved-casp-3 were examined in A2780/DDP and SKOV3/DDP cells transfected with si-NC, si-NEAT1 #2, si-NEAT1 #2 and anti-NC or anti-miR-770-5p at 72 h by Western blot. The difference was compared with the indicated control group and analyzed via ANOVA followed via Tukey post hoc test. *P<0.05.