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. 2020 Aug 14;12:7291–7303. doi: 10.2147/CMAR.S255808

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© 2020 Guo et al.

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The expression of circ_0013912 in human PDAC cells in vitro. (A) RT-qPCR assessed circ_0013912 expression level in PANC-1 and AsPC-1 cells, compared to normal human pancreatic duct epithelial cells HPDE-6/E6E7. (B, C) RT-qPCR assessed RNA expression of circ_0013912 and glyceraldehyde-phosphate dehydrogenase (GAPDH) in PANC-1 and AsPC-1 cells after RNase R treatment or not. (D, E) RT-qPCR assessed RNA expression of circ_0013912, GAPDH and U6 in cytoplasmic fraction and nuclear fraction of PANC-1 and AsPC-1 cells. (F) The schematic diagram showed siRNAs target splice junction of circ_0013912 (si-circ-1 and si-circ-2). (G) RT-qPCR assessed circ_0013912 expression level in PANC-1 and AsPC-1 cells transfected si-circ-1, si-circ-2 or the negative control si-NC. *P<0.05.