Table 1: “What happened?”.
Summary of transgenic tools used to lineage trace lung fibroblasts, combined with literature references and brief description of the finding. List of major mesenchymal lineages during lung development, repair, and homeostasis. Reports of similar and overlapping populations are grouped by lineage, transgenic tool and publication. A short description of when cells were labeled, and progenies documented.
| Lineage | Cell Type | Transgenic Mouse | Reference | Start | End |
|---|---|---|---|---|---|
| TBX4 | Myofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2016) (Kumar et al., 2014) (Zhang et al., 2013) |
E9.25 | E15.5 |
| Smooth Muscle Cell | Tbx4LME-CreER JAX # N/A (Kumar et al., 2014) | (Kumar et al., 2014) | E10.5 | E13.5 | |
| Pericyte | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2016) | E9.25 | E15.5 | |
| Endothelium | Tbx4LME -Cre JAX # 033331 (Greif et al., 2012) Tbx4-rtTA/TetO-Cre JAX # N/A (Zhang et al., 2013) |
(Xie et al., 2016) (Zhang et al., 2013) |
E9.25 | E15.5 | |
| (Zhang et al., 2013) | E11.5 | E15.5 | |||
| Mesothelium | Tbx4LME-CreER JAX # N/A (Kumar et al., 2014) | (Kumar et al., 2014) | E10.5 | E13.5 | |
| Lipofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2016) | E9.25 | E15.5 | |
| GLI1 | Myofibroblast | Gli1-CreERt2 JAX #007913 (Ahn and Joyner, 2004) | (Li et al., 2015) | E10.5–11.5 | E18.5 |
| P5–6 |
P11 P14 |
||||
| (Kugler et al., 2017) | P1 | P6, P10 | |||
| Mesenchymal Stem Cell | Gli1-CreERt2 JAX #007913 (Ahn and Joyner, 2004) | (Kramann et al., 2015) | 8-Week- | Two days | |
| Pericyte | Gli1-CreERt2 JAX #007913 (Ahn and Joyner, 2004) | (Li et al., 2015) | P5–6 | Adult | |
| Smooth Muscle Cell | Gli1-CreERt2JAX #007913 (Ahn and Joyner, 2004) | (Li et al., 2015) | E10.5–11.5 | E12.5, P11 | |
| (Li et al., 2015) | P5–6 | P11 | |||
| (Kugler et al., 2017) | P1 |
P6 P10 |
|||
| Mesothelium | Gli1-CreERt2 JAX #007913 (Ahn and Joyner, 2004) | (Li et al., 2015) | E10.5–11.5 |
P11 P14 |
|
| (Li et al., 2015) | P5–6 |
P11 P14 |
|||
| (Kugler et al., 2017) | P1 |
P6, P10 |
|||
| PDGFRa | Myofibroblast | C57/BL6 | (Bostrom et al., 2002; Bostrom et al., 1996) | N/A | E15.5 |
| PdgfrartTA;tetO-cre JAX # N/A (Li et al., 2018) | (Li et al., 2018) | E9.5–P7 | P7 | ||
| P0–P7 | P7 | ||||
| P1–P20 | P40 | ||||
| Pdgfra-creER™ JAX # 018280 (De Biase et al., 2011) | (Ntokou et al., 2015) | E9.5 |
E18.5, P5 P7 |
||
|
P2 P5 |
P7 P9 P14 |
||||
| PDGFRαEGFP JAX # 007669 (Hamilton et al., 2003) | (Endale et al., 2017) | N/A |
E16.5 E18.5 P7 |
||
| Matrix fibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | Adult | |
| PDGFRαEGFP JAX # 007669 (Hamilton et al., 2003) | (Endale et al., 2017) (Green et al., 2016) | PNX | Adult | ||
| Lipofibroblast | PdgfrartTA;tetO-cre JAX # N/A (Li et al., 2018) | (Li et al., 2018) | E9.5–P7 | P7 | |
| P0–P7 | P7 | ||||
| P1–P20 | P40 | ||||
| Pdgfra-creER™ JAX # 018280 (De Biase et al., 2011) | (Ntokou et al., 2015) |
E9.5 P2 P5 |
E18.5 P5 P7 P7 to P14 |
||
| Smooth muscle cell | PDGFRαEGFP JAX # 007669 (Hamilton et al., 2003) | (Ntokou et al., 2015) (El Agha et al., 2017) |
E9.5 | E14.5 to P3 | |
| FGF18 | Myofibroblast | FGF18CreERT2 JAX # N/A (Hagan et al., 2019a) Gli1LacZ JAX # 008211 (Bai et al., 2002) Gli1CreERT2 JAX # 007913 (Ahn and Joyner, 2004) |
(Hagan et al., 2019b) | PN5–8 | PN9, PN21 |
| PN1 |
PN2, PN7,
PN21 |
||||
| Gli1CreERT2 JAX # 007913 (Ahn and Joyner, 2004) Gli1LacZ JAX # 008211 (Bai et al., 2002) |
(Hagan et al., 2019b) | PN5–8 | PN9, PN21 | ||
| Lipofibroblast | FGF18CreERT2 JAX # N/A (Hagan et al., 2019a) Gli1LacZ JAX # 008211 (Bai et al., 2002) Gli1CreERT2 JAX # 007913 (Ahn and Joyner, 2004) |
(Hagan et al., 2019b) | PN5–8 | PN9, PN21 | |
| PN1 | PN2, PN7, PN21 | ||||
| Gli1CreERT2 JAX # 007913 (Ahn and Joyner, 2004) Gli1LacZ JAX # 008211 (Bai et al., 2002) |
(Hagan et al., 2019b) | PN5–8 | PN9, PN21 | ||
| Mesothelium | FGF18CreERT2 JAX # N/A (Hagan et al., 2019a) Gli1LacZ JAX # 008211 (Bai et al., 2002) |
(Hagan et al., 2019b) | PN5–8 | PN9, PN21 | |
| THY1 | Lipofibroblast | C57/B6; Thy1−/− JAX # N/A (Dr. Koger Morris, King’s College, London, UK) | (Varisco et al., 2012) | N/A | E18.5 to Adult |
| Mesenchymal Stem Cell | C57BL/6 | (McQualter et al., 2009) | Adult | Adult | |
| FGF10 | Lipofibroblast | Fgf10iCre JAX # 033807 (El Agha et al., 2012) | (El Agha et al., 2014) |
E11.5 E15.5 |
E15.5 E18.5 |
| Myofibroblast | Fgf10iCre JAX # 033807 (El Agha et al., 2012) | (El Agha et al., 2017) | Bleo Injury | Adult | |
| Smooth Muscle Cell | Fgf10iCre JAX # 033807 (El Agha et al., 2012) (Fgf10)-lacZ MGI:3629660 (Kelly et al., 2001) | (El Agha et al., 2014) | E11.5 | E13.5 to E18.5 | |
| (Mailleux et al., 2005) | E10.5 | E11.5 to E14.5 | |||
| Mesenchymal Stem Cell | Fgf10iCre JAX # 033807 (El Agha et al., 2012) | (El Agha et al., 2014) | E11.5 |
P30 Adult |
|
| TCF21 | Lipofibroblast | TCF21-creERT2 JAX # N/A (Park et al., 2019) | (Park et al., 2019) |
E9.5 11.5 15.5 |
E18.5 |
| Matrix Fibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | Adult | |
| Myofibroblast | TCF21-creERT2 JAX # N/A (Park et al., 2019) | (Park et al., 2019) | P2 | P7 | |
| Smooth Muscle Cell | TCF21-creERT2 JAX # N/A (Park et al., 2019) | (Park et al., 2019) |
E9.5 11.5 |
E18.5 | |
| PDGFKb | Pericyte | PDGFKb−/+ JAX # 007846 (Soriano, 1994) | (Greif et al., 2012; Hellstrom et al., 1999) | E11.5 |
E13.5, E18.5 |
| Smooth Muscle Cell | PDGFR-β-Cre JAX # N/A (Foo et al., 2006) | (Greif et al., 2012) | E11.5 | E13.5, E18.5 | |
| Lipofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | Adult | Adult After Injury | |
| Myofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | Adult After Injury | |
| (Henderson et al., 2013) | Adult | Adult After Injury | |||
| NG2 (CSPG4) | Pericyte | NG2-CreEK™ JAX # 008538 (Zhu et al., 2011) | (Rock et al., 2011) | Adult | Adult after injury |
| Myofibroblast | NG2-CreEK™ JAX # 008538 (Zhu et al., 2011) | (Rock et al., 2011) | Adult | Adult after injury | |
| Lipofibroblast | NG2-CreEK™ JAX # 008538 (Zhu et al., 2011) | (Rock et al., 2011) | Adult | Adult after injury | |
| FOXD1 | Pericyte | Foxd1+/GFPCreER JAX # 012464 (Humphreys et al., 2010) | (Hung et al., 2013) | E11.5 | E14.5, Adult |
| Myofibroblast | Foxd1+/GFPCreER JAX # 012464 (Humphreys et al., 2010) | (Hung et al., 2013) | Adult | Adult 7 days after injury | |
| Lipofibroblast | Foxd1-Cre JAX # 012463 (Humphreys et al., 2010) | (Hung et al., 2013) | E11.5 | Adult | |
| COL13A1 | Matrix Fibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | E16.5-Adult |
| Myofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | E16.5-Adult | |
| Lipofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | E16.5-Adult | |
| COL14A1 | Matrix Fibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | E16.5-Adult |
| Myofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | E16.5-Adult | |
| Lipofibroblast | Tbx4LME-Cre JAX # 033331 (Greif et al., 2012) | (Xie et al., 2018) | E9.25 | E16.5-Adult | |
| SCA-1 | Mesenchymal Stem Cell | Ly6aCre (Sca-1Cre) JAX #032621 (Vagnozzi et al., 2018) | (McQualter et al., 2009) | E18.5 | Adult |
| Myofibroblast | C57/BL6 | (Cao et al., 2018) | Adult | In Vitro After Injury | |
| Lipofibroblast | Ly6aCre (Sca-1Cre) JAX #032621 (Vagnozzi et al., 2018) | (McQualter et al., 2009) | E18.5 | Adult | |
| (Xie et al., 2018) | Adult | Adult | |||
| Matrix Fibroblast | PDGFRαEGFP JAX # 007669 (Hamilton et al., 2003) | (Green et al., 2016) | Adult | Adult after PNX | |
| (Xie et al., 2018) | Adult | Adult | |||
| Pericyte | Ly6aCre (Sca-1Cre) JAX #032621 (Vagnozzi et al., 2018) | (McQualter et al., 2009) | E18.5 | Adult | |
| Smooth Muscle Cell | Ly6aCre (Sca-1Cre) JAX #032621 (Vagnozzi et al., 2018) | (McQualter et al., 2009) | E18.5 | Adult | |
| ABCG2 | Mesenchymal Stem Cell | Abcg2CreERT2 JAX # 021961 (Fatima et al., 2012) | (Gaskill et al., 2017) | Adult | Adult |
| Pericyte | Abcg2CreERT2 JAX # 021961 (Fatima et al., 2012) | (Gaskill et al., 2017) | Adult | Adult, Adult after injury | |
| LGR5 | Alveolar Niche Cell | Lgr5EGFP-IRES-creERT2 JAX # 008875 (Barker et al., 2007) | (Lee et al., 2017) | Adult | Adult |
| Myofibroblast | Lgr5EGFP-IRES-creERT2 JAX # 008875 (Barker et al., 2007) | (Lee et al., 2017) | Adult | Adult, Adult After Injury | |
| AXIN2/WNT2 | Alveolar Niche Cell | Axin2CreERT2 JAX # 018867 (van Amerongen et al., 2012) Wnt2CreERT2 JAX # N/A (Peng et al., 2013) PDGFRαEGFP JAX # 007669 (Hamilton et al., 2003) |
(Zepp et al., 2017) | Adult | Adult |
| Myofibroblast | Axin2CreERT2 JAX # 018867 (van Amerongen et al., 2012) Wnt2CreERT2 JAX # N/A (Peng et al., 2013) PDGFRαEGFP JAX # 007669 (Hamilton et al., 2003) |
(Zepp et al., 2017) | Adult | Adult, Adult after injury | |
| LGR6 | Smooth Muscle Cell | Lgr6GFP-ires-CreERT2 JAX #016934 (Snippert et al., 2010) | (Lee et al., 2017) | Adult | Adult |
| Myofibroblast | Lgr6GFP-ires-CreERT2 JAX # 016934 (Snippert et al., 2010) | (Lee et al., 2017) | Adult | Adult | |
| WT1 | Mesothelium | Wt1CreERT2 JAX # 010912 (Zhou et al., 2008) | (Que et al., 2008) (Colvin et al., 2001) |
E10.5 | E11.5, E15.5, P10 |
| Smooth Muscle Cell | Wt1CreERT2 JAX # 010912 (Zhou et al., 2008) | (Que et al., 2008) | E10.5 | E15.5, P10 | |
| Myofibroblast | Wt1CreERT2 JAX # 010912 (Zhou et al., 2008) | (Que et al., 2008) | E10.5 | E15.5, P10 |
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