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Figure 1. Schematic illustration of the T-DNA region of the Platinum transcription activator-like effector nuclease (TALEN) expression construct. RB and LB, T-DNA right and left borders, respectively; 35SP-AtADH-5'UTR, Cauliflower mosaic virus 35S promoter 5'-untranslated region of A. thaliana alcohol dehydrogenase; RbcsT, Rubisco small subunit terminator; HSPT, heat shock protein terminator from Arabidopsis thaliana; NOSP, nopaline synthase promoter; NPTII, neomycin phosphotransferase II; NOST, nopaline synthase terminator. The double-headed arrows indicate the amplified regions in Figure 2.