Table 1. PCR primers and their applications.

Primer name	Primer sequence (5′→3′)	Application	Annealing temp. (°C)	Extention time (s)
pY365/1682/check-for	TGTTCTCTGACACTGTTGTAGCACT	Amplifying the SSR2 target site	55	30
pY366/1683/check-rev	TCGAAGCATACATACCGGTCATCAT
1748/StDWF1_C-Forward	TGTTCTCAGACACTGTTGTGTCATA	Amplifying the SSR1potential off target site	52	30
1749/StDWF1_C-Reverse	TTGAAGCATATCTACCAGTCATGCA
pY515/35SP-C-1F	AAGACTGGCGAACAGTTCATACAGAG	Detectting TALEN construct (35SP_1)	56	30
pY516/35SP-C-1R	GTCTTGCGAAGGATAGTGGGATTGTG
pY517/35SP-C-2F	CCCACAGATGGTTAGAGAGGCTTACG	Detecting TALEN construct (35SP_2)	55	30
pY518/35SP-C-2R	CTCTGTATGAACTGTTCGCCAGTC
pY519/TALN-C-1F	GAAGGACGCAAGTGGTTGGTCTAG	Detecting TALEN construct (TAL-N)	57	30
pY520/TALN-C_1R	CTCTGGCAACGCCGTGATTATGTG
pY27/FokI-for	CGCAAGAAATTCAACTCAGGATAGA	Detecting TALEN construct (FokI)	51	30
pY28/FokI-rev	CCGTTATTAAATTTCCTTCTCACTTCCT
1658/RbcsT-1-seq	TCCCCTTTCTGGAATATTCAGC	Detecting TALEN construct (RbcsT)	51	30
1659/RbcsT-400-rev-seq	CCAAATCTCCAATGGCTATGTC
pY521/HSPT-C_1F	GGCTTGTTGTGTTATGAATTTGTGG	Detecting TALEN construct (HSPT-NOSP)	52	40
pY522/NOSP-C_1R	GGAATTTATGGAACGTCAGTGGAGC
pY465/NPTII-F2	ATGATTGAACAAGATGGATTGCACGC	Detecting TALEN construct (NPTII)	55	60
pY466/NPTII-R2	TCAGAAGAACTCGTCAAGAAGGCG
pY523/NOSTU-C_1F	TATGAAAGGTTGGGCTTCGGAATCG	Detecting TALEN construct (NOST)	56	40
pY524/NOSTD-C_1R	CGAGATAGGGTTGAGTGTTGTTCCA