Table 2. Genome editing efficiency in regenerated shoots from Agrobacterium infected stem explants without selection.

Experiment	No. of infected stem explants	Co-cultivation time with Agrobacterium	No. of stem without Agrobacterium overgrowth	No. of isolated regenerated shoots	No. of HMA positive samples (crude genomic DNA, 1st assay)	No. of HMA positive samples (purified genomic DNA, 2nd assay)	No. of genome edited samples without transgene integration
1	100	3 days	100	67	5	3	1 (1.5%)
2	100	7 days	52	28	4	3	1 (3.6%)
3	200	6 days	200	132	11	7	2 (1.5%)
4	190	3 days	187	144	4	6	3 (2.1%)
Total	590	—	539	371	24 (6.5%)	19 (5.1%)	7 (1.9%)