Table 2. Copy number and segregation of the transgenes.
| Linesa | Copy number | GUS activity in T0 plants | PCR positive T1 plantsb | No. of locic | GUS positive T1 plantsb | ||
|---|---|---|---|---|---|---|---|
| uidA cassette (4.63-kb) | Other bands | Total | |||||
| Recovered from the 4% maltose condition | |||||||
| C1 | 4 | 5 | 9 | — | 40/48 | 1 | |
| C2 | 5 | 5 | 10 | — | 36/48 | 1 | |
| C3 | 8 | 3 | 11 | — | 26/48 | na | |
| C4 | 3 | 14 | 17 | + | 69/86 | 1 | 0/86 |
| C5 | 5 | 7 | 12 | + | 68/84 | 1 | 49/84 |
| C6 | 10 | 11 | 21 | + | 38/47 | 1 | 0/47 |
| P1 | 2 | 12 | 14 | — | 10/14 | 1 | |
| P2 | 0 | 12 | 12 | — | sterile | ||
| P3 | 0 | 9 | 9 | — | 46/57 | 1 | |
| P4 | 7 | 4 | 11 | + | 57/66 | 2 | 56/66 |
| P5 | 12 | 0 | 12 | + | 24/47 | na | 0/47 |
| P6 | 2 | 7 | 9 | + | sterile | ||
| Recovered from the 15% maltose condition | |||||||
| H1 | 0 | 5 | 5 | — | 39/49 | 1 | |
| H2 | 0 | 9 | 9 | — | 57/60 | 2 | |
| PH1 | 1 | 4 | 5 | — | 30/38 | 1 | |
| PH2 | 6 | 4 | 10 | — | 37/48 | 1 | |
| PH3 | 2 | 5 | 7 | — | 26/36 | 1 | |
| PH4 | 1 | 2 | 3 | + | 36/48 | 1 | 36/48 |
| PH5 | 1 | 2 | 3 | + | 25/47 | na | 25/47 |
| PH6 | 1 | 6 | 7 | + | 37/48 | 1 | 30/48 |
a Line names correspond to those in Figure 5. b Number of plants found to be positive for or express the transgene out of the total number of T1 plants analyzed. c Number of loci determined by the presence of transgene ratios with no significant difference from that expected, as determined using a χ2 test. na, not adapted to the Mendelian segregation patterns of 3 : 1 or 15 : 1.