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. 2020 Jun 25;37(2):141–146. doi: 10.5511/plantbiotechnology.20.0225a

Figure 2. Preparation of explants. Seed coats are removed using a scalpel and forceps. The peeled seeds are sterilized for 10 min in a 1% (w/v) sodium hypochlorite aqueous solution supplemented with one drop of Tween 20 with occasional agitation and then rinsed five times with sterile distilled water. The sterilized seeds are germinated at 28°C in dark for 1 day on the corresponding shoot-inducing medium. (A) A germinating cucumber seed, incubated in the dark for 1 day at 28°C. Scale bar=5 mm. (B) Detached cucumber cotyledons. The proximal regions marked with arrows are used as explants. Scale bar=5 mm. (C) Proximal region of squash cotyledon. Yellow arrow indicates the junction of the cotyledon and hypocotyl. Black arrowhead indicates shoot apex. Scale bar=1 mm. (D) Detached squash cotyledons. As in cucumbers, the proximal regions are used as explants. Scale bar=5 mm. All pictures are adapted from Nanasato and Tabei (2012) and Tabei and Nanasato (2012), courtesy of Kagaku-Dojin, Kyoto, Japan.

Figure 2. Preparation of explants. Seed coats are removed using a scalpel and forceps. The peeled seeds are sterilized for 10 min in a 1% (w/v) sodium hypochlorite aqueous solution supplemented with one drop of Tween 20 with occasional agitation and then rinsed five times with sterile distilled water. The sterilized seeds are germinated at 28°C in dark for 1 day on the corresponding shoot-inducing medium. (A) A germinating cucumber seed, incubated in the dark for 1 day at 28°C. Scale bar=5 mm. (B) Detached cucumber cotyledons. The proximal regions marked with arrows are used as explants. Scale bar=5 mm. (C) Proximal region of squash cotyledon. Yellow arrow indicates the junction of the cotyledon and hypocotyl. Black arrowhead indicates shoot apex. Scale bar=1 mm. (D) Detached squash cotyledons. As in cucumbers, the proximal regions are used as explants. Scale bar=5 mm. All pictures are adapted from Nanasato and Tabei (2012) and Tabei and Nanasato (2012), courtesy of Kagaku-Dojin, Kyoto, Japan.