Table 1. Efficiency of transgenic embryo selection at different concentrations of kanamycin.
Kanamycin concentration (mg l−1) | No. of AEC (A) | No. of AEC with GFP-fluorescent embryos (B)* | Percentage of AEC with GFP-fluorescent embryos (B/A×100) | No. of embryos developed (C)** | No. of GFP-Fluorescent embryos (D)** | Percentage of GFP-fluorescent embryos (D/C×100) |
---|---|---|---|---|---|---|
0 | 39 | 13 | 33.3 | 95 | 35 | 36.8 |
15 | 39 | 17 | 43.6 | 166 | 65 | 39.2 |
25 | 42 | 17 | 40.5 | 180 | 88 | 48.9 |
AEC: Aggregated embryogenic cells. * After a month of culture. **After 2 months of culture.