. 2020 Jun 25;37(2):147–156. doi: 10.5511/plantbiotechnology.20.0131a

Table 2. Composition of the COM medium.

Component	Stock solution (g l⁻¹)	Final concentration (mg l⁻¹)
COM-I stock solution (25×)^a
KNO₃	35.775	1,431
MgSO₄·7H₂O	10	400
CaCl₂·2H₂O	0.625	25
NaNO₃	7.75	310
NH₄H₂PO₄	5.625	225
COM-II stock solution (50×)^a
MnSO₄·4H₂O	0.18	3.6
H₃BO₃	0.4	8
ZnSO₄·7H₂O	1.25	25
KI	0.05	1
CuSO₄·5H₂O	0.12	2.4
Na₂MoO₄·2H₂O	0.01	0.2
CoCl₂·6H₂O	0.01	0.2
COM-III stock solution (50×)^{a, b}
Na₂–EDTA	2	40
FeSO₄·7H₂O	1.5	30
COM-IV stock solution (100×)^c
Thiamine–HCl	0.5	5
Nicotinic acids	0.5	5
Pyridoxine–HCl	0.05	0.5
COM-AA stock solution (20×)^{d, e}
Citrulline	0.395	19.75
Ornithine	0.38	19
Lysine	0.275	13.75
Alanine	0.2	10
Proline	0.175	8.75
Glutamine	11	550
Asparagine	10.2	510
Arginine	3.5	175
Other components
Myo-inositol		1,000
Maltose		30,000
Polyethylene glycol 4000		150,000
Activated charcoal		2,000
Gelzan CM		3,000
Abscisic acid ^e		26.4 ^f

^a Filter-sterilize and store at 4°C. ^b Dissolve each compound individually in about 200 ml of ddH₂O, add the FeSO₄·7H₂O solution little by little to the Na₂–EDTA solution, and then dilute to 1 l. ^c Store at −20°C. ^d Adjust stock solution to pH 5.7, filter-sterilize, and store at −20°C. ^e Add after adjusting the medium to pH 5.7 and autoclaving. ^f Final concentration is 100 µM.