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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Exp Cell Res. 2020 Jul 6;394(1):112165. doi: 10.1016/j.yexcr.2020.112165

Figure 2. Exogeneous TRAIL inhibits myoblast differentiation.

Figure 2.

(A) C2C12 cells were transduced with lentiviruses expressing shTRAIL or shScramble followed by 2 days of puromycin selection. Cells were then transfected with a mouse full-length TRAIL expression construct (TRAIL) or the corresponding empty vector (vector), selected with hygromycin, and differentiated for 72 hrs, followed by staining for MHC (green) and DAPI (magenta). Differentiation and fusion indices were quantified (n=4). Overexpression of TRAIL was confirmed by western blotting. (B) Cells treated as in (A) were cultured with 100 ng/mL sTRAIL or vehicle (control) for 6 to 9 hrs and then induced to differentiate for 72 hrs with sTRAIL present for the first 24 hrs, followed by staining for MHC (green) and DAPI (magenta). Differentiation and fusion indices were quantified (n=5). All error bars represent SEM. Two-tailed t-test was performed to compare each data to control (first bar in each graph). *P < 0.05; **P < 0.01.