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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Exp Cell Res. 2020 Jul 6;394(1):112165. doi: 10.1016/j.yexcr.2020.112165

Figure 6. TRAIL knockdown enhances muscle regeneration post-injury.

Figure 6.

(A) Mouse TA muscles were co-injected with BaCl2 and lentiviruses expressing shTRAIL or shScramble, isolated on Day 3 AI and subjected to RNA isolation and qRT-PCR (n=5). (B) TA muscles injected as in (A) were isolated on Day 7 or Day 14 AI and subjected to cryosection. Upon H&E staining, cross-sectional area (CSA) was quantified for regenerating myofibers (n=6 for D7AI, n=8 for D14AI). (C) TA muscles injected as in (A) were isolated on Day 3 AI, cryosectioned and immunostained for Ki-67 (red) along with DAPI (blue). The percentage of Ki-67 positive cells was quantified (n=5). All error bars represent SEM. Two-tailed t-test was performed to compare shTRAIL to shScramble. *P < 0.05; **P < 0.01. Scale bars: 50 μm.