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. 2020 May 22;123(4):591–603. doi: 10.1038/s41416-020-0899-2

Fig. 6. COMMD4 depletion leads to increased apoptosis in NSCLC cells.

Fig. 6

a Flow cytometry analyses of apoptosis with control siRNA and siRNA #2 and siRNA #3 treated HBEC3-KT, H1975 and CRL5889 cell lines. Live cells were stained with Annexin V-488 and propidium iodide and assessed using a cytoFLEX flow cytometer. The percentage of cells within each gating quadrant is listed for each panel. Live cells; lower left quadrant (Q4), apoptotic cells; right quadrants (Q2 and Q3). b, c. Quantification of (a). The percentage of necrotic (b) and apoptotic (c) cells is shown and was calculated from the quadrant. d Apoptosis measured by PARP cleavage by immunoblotting HBEC3-KT and NSCLC cell lines. e Quantification of (d). C; control siRNA, 2; COMMD4 siRNA #2, 3, COMMD4 siRNA #3. *p < 0.05, **p < 0.005, n.s; not significant. Error bars represent mean ± S.D from three independent experiments.