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. 2020 Aug 18;11(8):642. doi: 10.1038/s41419-020-02903-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Cell proliferation was detected by CCK-8 after treated with different concentrations of epirubicin in two BC cell lines. b, c DNER was analyzed by western blotting in BC cells treated as described above. Right: quantitative analysis of the optical density ratio of DNER compared with β-actin are shown. d Expression of epirubicin-induced DNER was detected by PCR. e Cell viability was assessed by CCK-8 after DNER knockdown treated with epirubicin or not. f Analysis of apoptosis with FACS in MDA-MB-468 cells treated as described in (e). Right: Quantitative analysis of apoptosis ratio. g The expression of PARP was detected by western blotting in BC cells treated as described above. Right: quantitative analysis of the optical density ratio of c-PARP compared with β-actin are shown. h Cell growth was measured by CCK-8 after DNER overexpression treated with epirubicin or not. i Analysis of apoptosis with FACS in MDA-MB-468 cells treated as described in (h). Right: Quantitative analysis of apoptosis ratio. j The expression of PARP was detected by western blotting in BC cells treated as described above. Right: quantitative analysis of the optical density ratio of c-PARP compared with β-actin are shown. The values are the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs the corresponding group.