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. 2020 Aug 18;11(8):642. doi: 10.1038/s41419-020-02903-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a β-catenin was analyzed by western blotting in BC cells treated with different concentrations of epirubicin. Right: quantitative analysis of the optical density ratio of β-catenin compared with β-actin are shown. b Expression of epirubicin-induced DNER detected by real-time RT-PCR. c, d Distribution of β-catenin in BC cells treated with epirubicin that were analyzed with confocal microscopy. β-catenin is stained green, and the nucleus is stained blue. Scale bar = 10 μm. e Cells were transfected with FLAG-NC or FLAG-DNER, and then treated with XAV-939 for 24 h before epirubicin treatment. Cell proliferation was detected by CCK-8. f Analysis of apoptosis with FACS in MDA-MB-468 cells treated as described above. Right: Quantitative analysis of apoptosis ratio. g The expression of PARP was detected by western blotting in BC cells treated as described above. Right: quantitative analysis of the optical density ratio of c-PARP compared with β-actin are shown. The values are the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs the corresponding group. h Schematic model for DNER-induced biological function of BC cells.