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. 2020 Aug 19;104(19):8427–8437. doi: 10.1007/s00253-020-10834-2

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© Springer-Verlag GmbH Germany, part of Springer Nature 2020

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Fig. 3 — Characterization of rescued viruses. (a) Amplification of IBV H120-specific cDNA fragments from different generations of rH120 and rH120/SCS1 passaged in SPF chicken embryonated eggs. 1–3, The first three generations of rH120. 4–6, The first three generations of rH120/SCS1. (b) H120, rH120, and rH120/SCS1 infected CEK cells detected by IFA with mAb against IBV M protein. (c) Western blot analysis of H120, rH120, and rH120/SCS1 infected chicken embryonic allantoic fluid with mAb against IBV M protein