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. 2020 Aug 12;10:1344. doi: 10.3389/fonc.2020.01344

Figure 2.

Figure 2

Differential expression of lnc-REG3G-3-1, miR-215-3p, leptin, and SLC2A5 and miRNAs in lung cancer cell lines and cellular localization of lnc-REG3G-3-1. Real-time RT-PCR was employed to confirm the expression differences of the gene levels of lnc-REG3G-3-1, leptin, and SLC2A5, and three miRNAs, including miR-215-3p, miR-5787, and miR-4505 in A549 and H1299 cells and the BEAS-2B cell line as control. The results are shown in (A,B). RNA FISH assay was used to detect the expression and localization results of lnc-REG3G-3-1 in H1299 cells (C). The abundant expression of lnc-REG3G-3-1 in the cytoplasm is pointed out by red arrows in the merged image. Dual-luciferase reporter assay was used to verify whether lnc-REG3G-3-1 has a specific adsorption effect on miR-215-3p in H1299 cells (D). The effects of the overexpression and knockdown of lnc-REG3G-3-1 on the expression levels of miR-215-3p, leptin, and SLC2A5 in H1299 cells were detected by real-time RT-PCR (E). Dual-luciferase reporter assay was used to verify whether miR-215-3p has a targeting effect on leptin and SLC2A5 in H1299 cells. The results showed that miR-215-3p has a targeting effect on leptin (F,a) and also with the same function on the SLC2A5 gene (F,b). Bar graphs represent the ratios plotted as mean ± SD of three separate experiments. Compared with the control group, *P < 0.05; **P < 0.01; ***P < 0.001.