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. 2020 Aug 12;10:1344. doi: 10.3389/fonc.2020.01344

Figure 3.

Figure 3

miR-215-3p effect on mRNA and protein expression of genes related to leptin and SLC2A5 signaling pathway. After the overexpression or knockdown of miR-215-3p by transfected plasmids, the mRNA expression levels of leptin, SLC2A5, Stat3, mTOR, JAK2, VEGF, AKT, Notch-1, PI3K, STYXL1, CUL7, FLAD1, and SOX4 were detected by real-time RT-PCR (A). Upregulation or downregulation of miR-215-3p by transfected plasmids and the protein expression levels of leptin, SLC2A5, Stat3, p-Stat3, JAK2, p-JAK2, VEGF, STYXL1, and FLAD1 were detected by Western blotting (B) and were analyzed the expression differences (C). GAPDH was used as a loading control. Representative blots were shown and repeated three times. Bar graphs represent the analysis of the ratios of each protein expression band density to that of GAPDH for nine genes. The ratios were plotted as mean ± SD of three separate experiments. Compared with the control group, “ns” means no statistical difference between means, *P < 0.05; **P < 0.01; ***P < 0.001.