Figure 4.

Reduction of lnc-REG3G-3-1 changed the growth of xenograft tumors in nude mice and gene expression levels. The average body weight and tumor volume of each group were obtained during modal mice feeding. The changes in body weight and the growth curve of the tumors are shown in (A,B). The dots of line charts represent the numbers plotted as mean ± SD (n = 6). After 21 days, the subcutaneous tumors were removed from each mouse separately (C). After modeling of nude mice xenograft tumors, the subcutaneous tumors were removed and the gene expression of lnc-REG3G-3-1 and miR-215-3p was detected by real-time RT-PCR (D). Western blotting (E,F) and immunohistochemistry (G) were used to detect the protein levels of leptin and SLC2A5 in tumor tissues. GAPDH was used as loading control. Bar graphs represent the analysis of the ratios of each target band density to that of GAPDH in each group for three genes in tumor tissues. The ratios were plotted as mean ± SD of three separate experiments. Compared with the control group, *P < 0.05; **P < 0.01; ***P < 0.001. For immunohistochemical results, representative photos are shown. Brown staining showed strong protein expression, whereas pale blue staining showed weak protein expression.