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. 2020 Aug 12;8:948. doi: 10.3389/fbioe.2020.00948

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Copyright © 2020 He, Cao, Xiang, Hu, Tang, Zhang, Albashari, Xing, Luo, Sun, Huang, Ye and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification and lineage differentiation of DPSCs. (A,B) Flow cytometry confirmed that DPSCs isolated and cultured in present study positively expressed surface markers of mesenchymal stem cells (CD73 and CD105), and negatively expressed surface markers of hematopoietic stem cells (CD34 and CD45). (C–E) Multiple differentiation potency of DPSCs used in this study was assessed by osteogenic, adipogenic and chondrogenic experiments. Fat droplets (red in C), calcified nodules (red in D), and chondrocytes (blue in E) were presented after 21 or 28 days inductions. Bar = 20 μm.