Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 18;11(8):654. doi: 10.1038/s41419-020-02886-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a qRT-PCR showing the relative expression of MyoD, Myogenin, MyH1 and Mical2 at day0 and day5 of C2C12 cell differentiation. b IF assay for MyHC (red) and MICAL2 (green) at day 0 and day 5 (C2C12) or day 2 (mSC) of skeletal muscle differentiation. Nuclei stained with HOECHST (blue). Scale bars 200 μm. c WB for MyHC and MICAL2 proteins on proliferating (day 0) and differentiating (day 5) C2C12 cells. The relative quantification is on the right. d IF analysis for MICAL2 (green) and LAMININ (red) localization on TA cross-sections of C57/Bl6 (C57). Scale bars 100 μm. N = 3. e WB for MICAL2 cytoplasmic and nuclear protein fractions on C57/Bl6 (C57) GN. The relative quantification is on the right. * = p < 0.05; ** = p < 0.01; *** = p < 0.001 by two-tailed t test. See also Figs. S1–S3.