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. 2020 Aug 18;11(8):654. doi: 10.1038/s41419-020-02886-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a qRT-PCR showing the relative expression of α-Sma, Sm22, Calponin, SM-MyHC, and Mical2 at day 0, day 2, day 5 and day 8 of smooth muscle differentiation. b IF assay for α-Smooth Muscle Actin (α-SMA; red) and MICAL2 (green). Nuclei stained with HOECHST (blue). Scale bars 50 μm. c WB and relative quantification for MICAL2 and α-SMA, on proliferating (day 0) and differentiating (day 8) mMABs. N = 3. d IF analysis for α-SMA (red) and MICAL2 (green) localization on gastrointestinal tract cross-sections of C57/Bl6 (C57). Scale bars 50 μm. N = 3. * = p < 0.05; ** = p < 0.01; *** = p < 0.001 one-way ANOVA test and two-tailed t test.