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. 2020 Aug 18;10:13946. doi: 10.1038/s41598-020-70787-4

Figure 2.

Figure 2

KIF11 is localized to the basal body of primary cilia in retinal pigmented epithelial cells and immortalized human neural progenitor cells. (a) Serum starved RPE1 cells were fixed and probed with α-Arl13b (green), α-Cep135 (green), α-KIF11 (red), and the DNA was counter-stained with Hoechst (blue). (b) Serum starved RPE1 cells were fixed and probed with α-Cep135 (green) and α-KIF11 (red) (left images) and α-Arl13b (green) and α-KIF11 (red) (right) to highlight KIF11 localization at the basal body. (c) Human neural progenitor cells were fixed and probed with α-Arl13b (green), α-Cep135 (green), α-KIF11 (red), and the DNA was counter-stained with Hoechst (blue). (d) Human neural progenitor cells were fixed and probed with α-Cep135 (green) and α-KIF11 (red) (left images) and α-Arl13b (green) and α-KIF11 (red) (right) to highlight KIF11 localization at the basal body. White boxes in (a) and (c) represent regions of interest shown in the additional panels.