Table 2.
Driving force of the redox (ΔE) and of the overall redox-coupled proton translocation (ΔG) reactions in States 3 and 4.
| State 3 | State 4 | ||||
|---|---|---|---|---|---|
| ΔE (mV) | ΔG (mV) | ΔE (mV) | ΔG (mV) | q | |
| (X2) | (ZX1 + X2) | (X2) | (ZX1 + X2) | ||
| Complexes I + III + IV | 1042 ± 5 (n = 3) | −192 | 1057 ± 9 (n = 3) | −123 | 0.880 |
| Complexes III + IV | 621 ± 4 (n = 3) | −111 | 643 ± 4 (n = 3) | −82 | 0.87 |
| Complex I | 384 ± 6 (n = 3) | −44 | 379 ± 4 (n = 3) | −5 | >0.99 |
| Complex III | 214 ± 9 (n = 4) | −44 | 222 ± 6 (n = 4) | −35 | 0.84 |
| Complex IV | 418 ± 15 (n = 5) | −78 | 434 ± 22 (n = 5) | −60 | 0.86 |
| (Complex IV) | 488 ± 15 (n = 5) | −148 | 504 ± 22 (n = 5) | −131 |
All numbers are in millivolts ± standard deviation. ΔP = 187 mV in State 4, 170 mV in State 3. The apparent q in State 4 is calculated according to Eq. (9). Complex IV data are calculated in two ways (see text and legend to Table 1). Values are expressed as mean ± SD, where n is the number of distinct samples.