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. 2020 Aug 3;11(8):648. doi: 10.1038/s41419-020-02792-4

Fig. 4. UPR suppression and increased cell death mediated by CAV1 is prevented by the S80A mutation.

Fig. 4

B16F10(Mock), B16F10(CAV1) or B16F10 cells expressing CAV1 mutant S80A (B16F10(CAV1/S80A)) were grown in the presence of IPTG (1 mM) for 48 h. a Total protein extracts were obtained and levels of CAV1 were evaluated by Western blotting and quantified. β-actin was included as a loading control (n = 3, mean ± SEM, two-way repeated measures ANOVA, **p < 0.01, ***p < 0.001). b B16F10(Mock), B16F10(CAV1) and B16F10(CAV1/S80A) were then exposed to increasing concentrations of Tm for 4 h (0.25–2 μg/μL). XBP1 mRNA splicing was detected and quantified as described (n = 3, mean ± SEM, two-way repeated measures ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001 between B16F10(CAV1) and B16F10(CAV1/S80A)). c The same cell lines described above were exposed to Tm (0.5 μg/μL) for 24 h and then cell death was evaluated following propidium Iodide staining (n = 3, mean ± SEM, two-way repeated measures ANOVA, *p < 0.05).