KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-calbindin D-128K | Millipore | Cat#AB1778; RRID: AB_2068336 |
| Rabbit anti-GAD65/67 | Millipore | Cat#AB1511; RRID: AB_90715 |
| Mouse anti-parvalbumin (clone PARV-19) | Sigma | Cat#P3088; RRID: AB_477329 |
| Mouse anti-somatostatin | GeneTex | Cat#GTX71935; RRID: AB_383280 |
| Rabbit anti-glial fibrillary acidic protein | Dako | Cat#N1506; RRID: AB_10013482 |
| Rabbit anti-Iba1 | Wako | Cat#019-19741; RRID: AB_839504 |
| Alexa Fluor 647-labeled goat anti-rabbit IgG | Thermo Fisher | Cat#A-21245; RRID: AB_2535813 |
| Alexa Fluor 647-labeled goat anti-mouse IgG | Thermo Fisher | Cat#A-21236; RRID: AB_2535805 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Thy1-G-CaMP: C57BL/6J-Tg(Thy1-G-CaMP7, -DsRed2)492Bsi | This paper | RIKEN BRC: RBRC06579 |
| Mouse: Shank2-deficient mice: B6N.129S4-Shank2tm1Mgle/CsbdJ | Won et al., 2012 | JAX: 033667; RRID: IMSR_JAX033667 |
| Oligonucleotides | ||
| Genotyping primer for Thy1-G-CaMP7 mice: CTGCTGCCCGACAACCA | Sato et al., 2015 | N/A |
| Genotyping primer for Thy1-G-CaMP7 mice: GTCGTCCTTGAAGAAGATGG | Sato et al., 2015 | N/A |
| Genotyping primer for Shank2-deficient mice: Shank2 WT fwd: GCTAGCATGACGTGTGTTGTG |
Won et al., 2012 | N/A |
| Genotyping primer for Shank2-deficient mice: Shank2 KO fwd2: CCGACTGCATCTGCGTGTTC |
Won et al., 2012 | N/A |
| Genotyping primer for Shank2-deficient mice: Shank2 rev: ACCTGTGTGTGATTTCTGAC | Won et al., 2012 | N/A |
| Recombinant DNA | ||
| Mouse Thy1.2 promoter | Feng et al., 2000 | RRID: Addgene_20736 |
| G-CaMP7-T2A-DsRed2 | Ohkura et al., 2012 Sato et al., 2015 | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | RRID: SCR_003070 |
| TurboReg | Biomedical Imaging Group, Swiss Federal Institute of Technology Lausanne | RRID: SCR_014308 |
| MATLAB | Mathworks | RRID: SCR_001622 |
| LabVIEW | National Instruments | RRID: SCR_014325 |
| OmegaSpace | Solidray | http://www.solidray.co.jp/product/omega/index.html |
| Prism | GraphPad | RRID: SCR_002798 |
| Modified nonnegative matrix factorization | Takekawa et al., 2017 | https://www.biorxiv.org/content/10.1101/215145v1 |
| Constrained nonnegative matrix factorization |
Pnevmatikakis et al., 2016 Giovannucci et al., 2019 |
https://github.com/flatironinstitute/CaImAn |
| Cell registration | Sheintuch et al., 2017 | https://github.com/zivlab/CellReg |