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. 2020 Aug 12;11:1872. doi: 10.3389/fmicb.2020.01872

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Copyright © 2020 Sullivan, Allen, Atkins, Chung, Dampier, Nonnemacher and Wigdahl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determinants of on‐ and off-target activity of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) based therapeutics. The two main components of the CRISPR/Cas9 system are the Cas9 endonuclease and the guide RNA (gRNA). Specific structural alterations of these components have been shown to enhance the on-target excision rate and/or reduce the off-target excision rate. Here, we present some of the factors that influence these excision rates.