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. 2020 Aug 12;10:1261. doi: 10.3389/fonc.2020.01261

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Copyright © 2020 Xing, Tian, Zhang, Guo, Tang, Wang, Lv and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The impact of DDX39 downregulation on the proliferation, invasion, and migration of melanoma cells was investigated by RNA interference mediated by lentivirus. The effect of DDX39A knockdown on the cell proliferation of A375 (A) and SKMEL28 (B) cells was measured by the Celigo Cell Counting application system. Beginning on day 2, the cells were imaged and counted daily for 5 days. The effect of DDX39A knockdown on the cell growth of A375 (C) and SK-MEL28 (D) cells was assessed by the MTT experiment in triplicate daily for 5 days. The mean and standard deviation (SD) values of at least three repeated experiments are reported. ***P < 0.001, compared with the shCtrl group.