Figure 2.
Loss and recovery of 2W1S-specific memory CD4 T cells following CLP-induced sepsis. (A) Experimental design—B6 mice were immunized with attenuated Listeria monocytogenes-2W1S (107 CFU i.v.) 30 days before sham or CLP surgery. The number of 2W1S-specific CD4 T cells were determined after surgery. (B) Survival of LM-2W-infected B6 mice after sham and CLP surgery (n = 29 sham; n = 67 CLP). The number of (C) total CD4 T cells and (D) 2W1S-specific CD4 T cells in the spleen was determined 2, 7, 14, and 28 days after sham or CLP surgery by flow cytometry. In addition, the 2W1S-specific CD4 T cells were subtyped based on (E) Tbet (Th1 phenotype) and (F) Foxp3 (regulatory T cell phenotype) expression. Data shown are cumulative from 3 independent experiments, with at least 3 mice/group/time point in each experiment. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005, and ****p ≤ 0.001.