Figure 4.

Sepsis impairs the recall response by pre-existing 2W1S-specific memory CD4 T cells to cognate Ag. (A) Experimental design—B6 mice were immunized with attenuated L. monocytogenes-2W1S (10⁷ CFU i.v.) 30 days before sham or CLP surgery. The mice were given a second infection with attenuated L. monocytogenes-2W1S (10⁷ CFU i.v.) (B) 2 or (C) 30 days after surgery. Total number of 2W1S-specific CD4 T cells in the spleen was determined the day of and 7 days after the second LM-2W1S infection (10⁷ CFU i.v.). The fold increase in cell numbers at day 7 post-secondary infection is indicated. Data shown are representative of 2 independent experiments, with 4 mice/group in each experiment. **p ≤ 0.01.