Figure 1.

Bioanalyzer traces showing the effect of AMPure bead ratio on library fragment size distribution. Traces represent analyses of a pool of four Clinical Virology Multiplex I panel (CVM panel) libraries, two of which were digitonin-DNAse treated and two untreated. The pool was initially cleaned using a 1.8X AMPure bead ratio (open circles), before being cleaned a second time using a 0.85X AMPure bead ratio (closed circles). The x-axis time variable correlates with library fragment size – earlier fragments are shorter than later ones. The peaks at 43 and 113 s are internal control fragments of known molecular weight.