Table 1.
Reduction of human DNA in cerebrospinal fluid (CSF) extracts following digitonin-DNAse treatment of three sample sets.
| Digitonin conc. (μg/ml) | Threshold cycle (Ct) | Total DNA conc. (ng/μl) | ||
|---|---|---|---|---|
| c-myc | β-globin | |||
| Control CSF | 0 | 31.5 | 32.2 | 1.23 |
| 25 | 35.5 | 36.4 | 0.15 | |
| 50 | >40 | >40 | <0.10 | |
| 75 | 36.4 | >40 | 0.13 | |
| 100 | >40 | >40 | 0.17 | |
| Control CSF spiked with CVM I Panel | 0 | 30.2 | 29.4 | 1.95 |
| 30.3 | 29.4 | 2.12 | ||
| 50 | 35.9 | 35.9 | <0.10 | |
| 35.7 | 35.8 | 0.10 | ||
| Patient CSF samples | 0 | 29.0 | 28.6 | 1.6 |
| 28.4 | 28.2 | 1.8 | ||
| 27.3 | 27.5 | 3.45 | ||
| 33.6 | 34.2 | <0.10 | ||
| 50 | 32.1 | 32.2 | 0.22 | |
| 33.9 | 34.0 | <0.10 | ||
| 30.3 | 30.0 | 0.56 | ||
| 32.5 | 33.5 | <0.10 | ||
DNA concentrations were determined by Qubit analysis.