Fig. 5. The variant at position 28,958,437 bp in Can-0 modulates FLM function and may play a role in adaptation.

a Accumulation of exon2-containing isoforms (typical of FLM-β) measured by qRT-PCR using the primer pair qFLM-F3 + qFLM-R3b (see Fig. 2) in nine Arabidopsis strains from the Iberian Peninsula (IP) carrying either the Can (blue) or the Col (brown) genotype at position 28,958,437 bp. Each data point represents one IP strain. The asterisks are the mean expression of each variant. b Boxplots represent leaf production rate with or without vernalization in Iberian Peninsula strains categorized according to their genotype at position 28,958,437 bp. The data were obtained from a previous publication⁴² and are gathered in Supplementary Data 3. Significance of the differences was calculated according to a mixed-effect model using the genotype as a random factor and the kinship matrix as covariates (see Supplementary Table 1). c Boxplots represent isothermality in IP strains categorized according to their genotype at position 28,958,437 bp. Isothermality is calculated as follows: mean of monthly (max temp − min temp)/(max temperature of warmest month − min temperature of coldest month) × 100, and was obtained from http://www.worldclim.org/ and are gathered in Supplementary Data 3. Significance of the differences was calculated according to a mixed-effect model using the genotype as a random factor and the kinship matrix as covariates (see Supplementary Table 1). Source data underlying Fig. 5a are provided as a Source Data file.