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. 2020 Aug 18;10:13935. doi: 10.1038/s41598-020-70965-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Box plot comparisons of C_q values for the seven candidate reference genes in honey bee samples. Samples were prepared from four seasons (A–D), three tissue types (E–G) and an integration of all samples (H). The horizontal lines in the box indicate the 25th, 50th and 75th percentile values. The square symbol in the big box shows the mean median. The error bars denote the maximum and minimum values.